Introduction: Regulated exocytosis
نویسندگان
چکیده
منابع مشابه
Introduction: Regulated exocytosis
Calcium ions regulate secretory processes in several ways. Most prominently they (i) trigger the release of vesicle contents rapidly and in a highly cooperative way and they (ii) control priming steps, which prepare vesicles for release. The importance of using assays with high time resolution for separating these distinct roles is pointed out here. vailable online xxx
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Kinesin and myosin have been proposed to transport intracellular organelles and vesicles to the cell periphery in several cell systems. However, there has been little direct observation of the role of these motor proteins in the delivery of vesicles during regulated exocytosis in intact cells. Using a confocal microscope, we triggered local bursts of Ca 2 1 -regulated exocytosis by wounding the...
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Real-time imaging of regulated exocytosis in secreting organs can provide unprecedented temporal and spatial detail. Here, we highlight recent advances in 3D time-lapse imaging in Drosophila salivary glands at single-granule resolution. Using fluorescently labeled proteins expressed in the fly, it is now possible to image the dynamics of vesicle biogenesis and the cytoskeletal factors involved ...
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ژورنال
عنوان ژورنال: Cell Calcium
سال: 2012
ISSN: 0143-4160
DOI: 10.1016/j.ceca.2012.04.013